In vitro evaluation of oocyte nuclear maturation in young and adult domestic cats (Felis catus)
DOI:
https://doi.org/10.1590/S1413-95962004000400008Keywords:
Domestic cat, Follicles, Nuclear maturationAbstract
This study assessed the in vitro oocyte nuclear maturation in adult and young domestic cats. Fifteen ovaries were used; 10 from adult females and 5 from young females. The oocytes collected by aspiration were quantified and classified. The oocytes classified as excellent and/or regular were grouped (10 oocyte /drop) in culture medium covered with mineral oil in disposable Petri dishes. The oocyte were incubated at 38°C and 5% de CO2 for 48 hours and then washed with 0,4% hyaluronidase, fixed in methanol/acetic acid and stained with acetic orcein. Evaluation of chromosomal configuration of oocytes matured in vitro showed 44,68% of oocyte in metaphase II in the adult female group and 25,32% in the young donor group, showing that puberty influences oocyte capacity for in vitro development.Downloads
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Published
2004-08-01
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How to Cite
1.
Lopes MD, Uiechi E, Trinca LA. In vitro evaluation of oocyte nuclear maturation in young and adult domestic cats (Felis catus). Braz. J. Vet. Res. Anim. Sci. [Internet]. 2004 Aug. 1 [cited 2024 May 4];41(4):268-73. Available from: https://www.revistas.usp.br/bjvras/article/view/6287